Examples of Mapping Color Space Sequences to 3GB Human Genome
These results were reported to us by Applied Biosystems (Foster City), using just one
of the ISAS / GenomeCruncher (2.4GHz  8 core) systems they had purchased from us.
Run times can be faster on more powerful servers, or slower on inferior ones.
Run Description
Total
Bases
Total Run
Time
Uniquely Mapped
and AAA for matepair
200 Million 25mers with 2 substitutions
5G
   
400 Million 25mers (200M+200M matepairs)
4 substitutions per 50mer pair
10G
104 minutes
61.0%
500 Million 50mers with 5 substitutions
25G
2.5 hours
41.0%
1 Billion 50mers (500M+500M matepairs)
10 substitutions per 100mer pair
50G
7.5 hours
33.1%
Alignment Mode
Alignment Rate (Reads/Hour)
First rate does not include writing
SAM output file. Second rate does.
Mapped
Uniquely
Mapped
35mers 1 substitution
1.81 Billion or 1.01 Billion
94.1%
71.8%
35mers 1 substitution, insertion, or
deletion (gapped)
     
60mers 2 subs
905 Million or 609 Million
90.8%
76.1%
60mers 2 subs, ins, or dels (gapped)
     
75mers 3 subs
511 Million or 401 Million
89.5%
76.2%
75mers 3 subs, ins, or dels (gapped)
     
88mers 4 subs
352 Million or 294 Million
84.5%
73.6%
88mers 4 subs, ins, or dels (gapped)
     
Examples of Mapping Illumina Sequences to 3GB Human Genome
We ran data supplied by NCGR on one ISAS / GenomeCruncher (2.4GHz  8 core)